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KMID : 0378019810240060107
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New Medical Journal
1981 Volume.24 No. 6 p.107 ~ p.112
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The Effect of Parathyroid Tissue Culture on Xenograft Survival in Rats
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Abstract
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Many surgeons, including William Halsted(1909), have attempted the parathyroid gland transplantation in animal models and men over 80 years, but it´s results were variable.
There is still an urgent clinical need for parathyroid transplantation in patients with idiopathic hypoparathyroidism as well as in more common postoperative iatrogenic
hypoparathyroidism that can not be managed with exogenous calcium and vitamin D therapy. If such a procedure were available, inadvertant post-operative hypoparathyroidism would benefit, and radical surgery for thyroid and laryngeal carcinoma could be performed more completely and with less morbidity.
Recent reports showed that culture of endocrine tissue before allograft results in prolonged survival (Talmage, 1976; Lafferty et al., 1975; Starling et el., 1977; Sollinger et al., 1977).
The purpose of this study was to observe the effect of donor tissue culture before transplantation on graft survival in xenograft (human parathyroid adenoma to Fisher rat).
The donor tissues were minced finely (1-2mm3), and placed on tissue paper on stainless steel grids over media well in organ culture dishes (Falcon) with plexiglass boxes filled with 95% 02-5% C02 in room temperature.
Standard culture medium-consisted of minimal essential medium (MEM) (GIBCO) supplemented with 10% fetal calf serum, aqueous penicillin-G(1, 000u/dl), streptomycin (1,000 fig/dl), fungizone(25μg/dl), non-essential amino acids (X 100) (1.Oml/dl) and L-glutamine (lOmM/d/).
Routine culture media changes were done 3 times per week.
The parathyroid tissues were transplanted into the ´thingh muscle after 3 weeks culture
to the hypocalcemic (below 5.5mg%)-Fisher recipient rats. The experimental results were as follows:
1. The mean serum calcium level of normal Fisher rat was 9.5±1.09mg%.
2. The total parathyroidectomized rats were followed up to 8 weeks in 7 rats, and there were no spontaneous recovery.
3. The survival rate for 8 weeks was 53.8% in cultured group, and 11.1% in non-cultured group.
The average survival days was 41.6±19.2 days in cultured group, and 15.0±16.9 days in non-cultured group.
There was significant prolongation of graft tissue survival in cultured group (p<0.01).
4. This tissue culture method could be useful in prolongation of parathyroid xenograft survival and useful method for preservation of endocrine tissue also.
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KEYWORD
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